Aims: To study the effect of low dose gamma irradiation on the sterility of Indian honey. Methods: A newly arrived, freshly obtained, raw unprocessed honey, available at Amit Jain’s centre for Apitherapy at Amit Jain’s Institute of diabetic foot and wound care, Brindhavvan Areion hospital, Bengaluru, was used in this study. This unsterilised honey was cultured in the microbiology laboratory of RRMCH. The honey samples were then subjected to 2.5, 5 and 20KGy gamma irradiation at IIHR, Bengaluru that has the cobalt 60 gamma irradiation chamber facility. Results: The unsterilised Indian honey had yielded aerobic spore forming Gram-positive bacilli and bacteriodes species. After irradiation of the three samples with different dose of gamma irradiation, it was noted that honey, which received a dose of 2.5KGy and 5Kgy, grew the same organisms. Conclusions: This study, which was aimed to determine the sterility with low dose gamma irradiation, found 2.5KGy and 5KGy irradiation doses to be ineffective in achieving sterilisation of Indian honey.