Background: An extracellular matrix graft (OASIS wound matrix, OASIS) reportedly provides an improved environment for chronic wound healing. This study aimed to evaluate the effectiveness of the extracellular matrix graft for chronic limb-threatening ischaemia (CLTI) in histopathological examination. Methods: The extracellular matrix graft was applied to half of the wound postsurgery; the other half served as the control. The granulation tissues were biopsied after one week and on the reconstruction day. The cells were counted during tissue examination. Results: Seven patients achieved complete healing. In 1 week, the extracellular matrix graft group had more cells than the control group (p<0.05). Conclusions: The extracellular matrix graft improved wound healing in CLTI.